Development and Validation of Analysis Method For Anthraquinone by liquid Chromatography Tandem Mass Spectrometry
ABSTRACT: Indonesia is one of
the largest tea producer and exporter in the world. However, the European Union
has issued Commission Regulation (EU) No.1146/2014 by setting the MRL (maximum
residue limit) of anthraquinone in tea at 20 μg/kg, significantly limiting the
amount of tea export from Indonesia to the European Union. Viewed from the side
of the danger, especially for synthetic anthraquinone that is potential effect
of human health then performed an analysis to determine the presence of
anthraquinone in Indonesian tea. To find out anthraquinone concentrations in
various tea products, anthraquinone was developed and validated analysis method
using LC-MS/MS. anthraquinone was extracted from tea sample using
dichlormethane with a soxhlet. Black tea and green tea samples were obtained
from one of the tea plantation companies.The validated method was used for the
determination of anthraquinone residues in black tea and green tea samples.
Anthraquinone residues was detect in all of the test samples.
Keyword: anthraquinone; liquid
chromatography-tandem mass spectrometry; green tea; black tea; validation
Penulis: Deddi Prima Putra, Amri
Bakhtiar, Prima Hajatri
Kode Jurnal: jpfarmasidd170145
