EFEK KO-KEMOTERAPI FRAKSI ETIL ASETAT AKAR PASAK BUMI DAN DOXORUBICIN TERHADAP PROLIFERASI DAN EKPRESI BAX JARINGAN PAYUDARA TIKUS SD
Abstract: The use of
co-chemotherapy agent was needed since there were some toxicities on the normal
tissues caused by the use of doxorubicin. The root of Eurycoma longifolia Jack,
a part of plant, has a potential activity as co-chemotherapy. This study aimed
to determine the effect of co-chemotherapy of ethyl acetate fraction of E.
longifolia Jack roots and doxorubicin against cell proliferation activity by
AgNOR method and determine expression of Bax protein of breast tissue in rats
induced by DMBA. The rats were divided into five groups: I (baseline), II (DMBA
20 mg/kgBW), III (DMBA, doxorubicin 1.12 mg/kg), IV (DMBA, fraction of 100
mg/kg), and V (DMBA, doxorubicin, fractions). All the rats were sacrificed at
week 16. Their breast tissue was evacuated. Cell proliferation and expression
was observed using AgNOR method and immunohistochemistry, respectively. Results
showed the percentage of p-AgNOR obtained by the healthy group, DMBA group,
DMBA+Doxorubicin group, DMBA+ethyl acetate fraction of E. longifolia Jack root
group, and DMBA+Doxorubicin+Ethyl acetate fraction of E. longifolia group were
0±0%, 14.67±2.11%, 1.83±1.21%, 6.83±2.03%, and 4.08±0.95%, respectively. The
percentage of Bax expression obtained by the healthy group, DMBA group,
DMBA+Doxorubicin group, DMBA+ethyl acetate fraction of E. longifolia Jack root
group, and DMBA+Doxorubicin+Ethyl acetate fraction of E. longifolia group were
68.82±1.52%, 26.86±3.25%, 44.49±2.06%, 80.92±3.27%, and 78.70±4.87%,
respectively. Based on the results, it was concluded that co-chemotherapy agent
of ethyl acetate fraction of E. longifolia Jack roots and Doxorubicin could
inhibit proliferation and trigger apoptosis through Bax expression in breast
tissue of rats induced by DMBA.
Keywords: Eurycoma longifolia
Jack root; Bax; co-chemotherapy; proliferation
Penulis: Tria Zakinah, Laela
Hayu Nurani, Sitarina Widyarini
Kode Jurnal: jpfarmasidd170441
