Efek Nanopartikel PLGA Ekstrak Biji Nigella sativa terhadap Kadar Katalase Hepar Tikus Model Diabetes Melitus Tipe 2
Abstract: Nigella sativa (NS),
a medicinal plant, has antidiabetic and antioxidant effects via regulating
blood glucose and antioxidant defence level. One of the antioxidant defence
capacity is catalase (CAT) which prevents oxidative stress in the DM
progression via balancing redox state. This enzyme could be increased by
Nigella sativa seeds extract (NSE). However, plant-derived product has limited
efficacy in clinical trial. Thus one of drug delivery system, PLGA encapsulated
nanoparticle, may improve this efficacy. Objectives: The aims of this study
were to evaluate CAT level in the liver tissues of male DM Sprague dawley rats
after oral administration of NSE 48 mg/kgBW/day (P1), PLGA nanoparticle of NSE
48 mg/kgBW/day (P2), glibenklamide 0,45 mg/kgBW/day (PP), and normal diet
without therapy (DN). Methodology: True experimental design post test
controlled group, 24 rats were fed by high-fat diet for 40 days and followed by
intraperitoneal injection of 35 mg/kgBW STZ. These rats were divided into 4
groups: DN, PP, P1, and P2 respectively. Results: Thymoquinone which possesses
antioxidant effect was identified by qualitative phytochemical evaluation. PLGA
nanoparticle was prepared from nanoprecipitation. The oral administration of
PLGA nanoparticle NSE for 26 days to DM rats lowered fasting blood glucose
(FPG) (66,67%) better than P1, PP, and DN. The PLGA nanoparticle of NSE did not
significantly increased the CAT level in the liver tissues of DM rats (analyzes
Oneway-ANOVA p = 0.872). However, the mean of CAT level in the liver tissues of
DM rats was higher in group P2 compared to those in the other group.
Conclusion: These results exhibited antidiabetic and antioxidant effects of
PLGA nanoparticle NSE in term of fasting
blood glucose level and CAT level in
liver tissues.
Key words: Nigella sativa,PLGA
Nanoparticle, Catalase
Penulis: pratiwi oktaviana
Kode Jurnal: jpfarmasidd160532
