EFFECT OF ETHANOL EXTRACT OF Eleutherine bulbosa (MILL.) URB ON ANAEROBIC BACTERIAL Prophyromonas gingivalis IN VITRO
Abstract: Periodontitis is an
inflamation of supporting tissues on teeth that became the main cause of
Prophyromonas gingivalis (P. gingivalis) bacteria. Treatment of periodontitis
is by giving antibacterial agent therapy. Plants that have antibacterial
effects one are Eleutherine bulbosa (UEB) bulbs from the Iridaceae family. This
plant has been used for ethnobotany boils drugs and has been known to have
antibacterial activity toward against intestinal pathogen bacteria that is
anaerobic Gram Negative, but still unknown its activity against on oral
pathogenic bacteria such as P. gingivalis. The purpose of this study was to
know the effect of UEB extract on the growth of P. gingivalis bacteria as in
vitro. UEB is taken from agriculture center in Samarinda city. UEB Simplisia
was tested for antibacterial activity using Kirby-Bauer disc diffusion method
on BHI-A media supplemented with vitamin K and hemin. The bacteria which used
were P. gingivalis ATCC® 33277. Blank disc 6 mm with nine concentrations
attached to BHI-A medium, incubated for 24 hours at 37 °C in an anaerobic
atmosphere, then sprayed MTT (3-[4,5-Dimethylthiazole-2-yl]-2,5-diphenylttetrazoliumbromide)
reagent and read a few moments later. For comparison, then used Chlorhexidine
gluconate (CHX) 2 mg/ml. The statistical analysis with t-test and there was
significant different if p<0.05. The
result showed that the higher concentration of UEB ethanol extract will
increase the growth inhibition zone of P. gingivalis bacteria and reach maximum
at 10 mg/ml concentration. The result of t-test showed no difference
significant of P. gingivalis bacterial inhibition zone in the treatment group
of 7.5 mg/ml and 10 mg/ml concentration on CHX group. This study proves UEB
ethanol extract can inhibit the growth of P. gingivalis bacteria.
Keywords: Eleutherine bulbosa,
Prophyromonas gingivalis, Inhibition Zone, Antibacterial Agents, Periodontitis
Penulis: Siti Nur Azizah, Sinar
Yani, Sjarif Ismail, Masyudhi, Endang Sawitri
Kode Jurnal: jpfarmasidd180035
