FORMULASI MIKROEMULSI EKSTRAK TERPURIFIKASI DAUN BAYAM MERAH (Amaranthus tricolor L.) SEBAGAI SUPLEMEN ANTIOKSIDAN
Abstract: A research has
conducted about the microemulsion formulation of purified extract of red
spinach leaves (Amaranthus tricolor L.) as an antioxidant supplement with the
aim to be able to know the formula to form a microemulsion which meet the
physical quality stability and determine antioxidant activity (IC50) of the
preparation. Extracts prepared by maceration method using ethanol 96% and then
later do the purification using solvent n-hexane and ethyl acetate, after it
tested its antioxidant activity. Formula to form a microemulsion purified
extract of red spinach leaves clear is to use virgin coconut oil (VCO) by 15%,
tween 80 for 40%, 35% glycerin and 10% distilled water. Physical stability test
was conducted on the organoleptic test, measuring the diameter of globules, pH
test, test and test viscosity centrifugation. Test of antioxidant activity in
vitro using DPPH method and using Vitamin C as a positive control. Measurement
data were statistically analyzed using paired samples T test. The test results
of antioxidant activity microemulsion purified extracts of spinach leaves, red
show IC50 values on day 1 was 1.83 ppm and the 28th day amounted to 3.71 ppm.
While vitamin C microemulsion shows IC50 values on day 1 of 0.24 ppm and the
28th day of 2.51 ppm. Despite the decreased antioxidant activity, but each of
the stocks included in the category of very powerful antioxidants.
Keywords: Microemulsion;
Amaranthus tricolor L.; physic stability; DPPH
Penulis: Dwi Lestari
Handayani, Yusriadi, Ririen Hardani
Kode Jurnal: jpfarmasidd170553