FRAKSI ETIL ASETAT KULIT BATANG FALOAK (Sterculia quadrifida R.Br ) MENGINDUKSI APOPTOSIS DAN SIKLUS SEL PADA SEL KANKER PAYUDARA T47D
Abstract: Faloak (Sterculia
quadrifida R.Br) is one source of bioactive compounds that could be developed
as chemotherapeutic agent. Empirically East Nusa Tenggara people use boiled
water of faloak bark as a cure for hepatitis (types A, B, and C), and
gastroenteritis. This study was performed to test anticancer activity fraction
of n-hexane, ethyl acetate, ethanol, and methanol of ethanolic extract from
faloak stem bark for the type of breast cancer cell line T47D, and normal cell
types Vero using cytotoxic 3- (4,5-dimetilazol- 2-yl) -2,5-difeniltetrazolium
bromide (MTT) test method. Ethanolic extract was subjected to column
chromatography using different solvents polarity level as n-hexane, ethyl
acetate, ethanol, and methanol. Testing the cytotoxic effects using the MTT
assay in T47D breast cancer cells and normal Vero cells with EC50 parameter.
Ethyl acetate fraction in inducing apoptosis and cell cycle modulation was
observed with flowcytometry method. The test results cytotoxic fraction
indicating the fraction of ethyl acetate has the lowest activity with EC50 of
24.88 μg/mL and selectivity index of 15.58. Ethyl acetate fraction effects an
accumulation of cells in S phase (27.43%) in breast cancer cells T47D which is
able to induce apoptosis. These results demonstrate that the ethyl acetate
fraction can be developed as a chemotherapeutic agent in improving the effectiveness
of breast cancer treatment.
Keywords: apoptotic; breast
cancer; cell cycle; cytotoxicity; faloak
Penulis: Rollando, Kestrilia
Rega Prilianti
Kode Jurnal: jpfarmasidd170439
