IDENTIFIKASI DAN PENETAPAN KADAR RHODAMIN B DALAM KERUPUK BERWARNA MERAH YANG BEREDAR DI PASAR ANTASARI KOTA BANJARMASIN
Abstract: Crackers are made
from tapioca flour batter mixed with flavorings and colorings, still many
outstanding crackers that contain ingredients banned dye Rhodamine B. Rhodamine
B is a chemical used for red dye in the textile industry and plastic. Rhodamine
B can cause cancer, poisoning, lung irritation, sore eyes, and sore throat.
This study aims to identify and determination the levels of Rhodamine B in
circulating red crackers Antasari market Banjarmasin.The population is that
sold in the red crackers that sold in Antasari market Banjarmasin.. The
sampling is technique incidental sampling , that is based on chance, so any
population by chance met with researchers can be used as a sample.
Identification of Rhodamine B was done by Thin Layer Chromatography (TLC) by
using the stationary phase silica GF 254 and mobile phase is elution solvent is
n-butanol, ethyl acetate, ammonia (10:4:5). Then detected with a UV lamp 254 nm
and 366 nm. While for the determination of levels using Vis spectrophotometry
at a wavelength of 544 nm.The results showed that the samples of 6 found one
sample containing Rhodamine B, namely samples 5 (cassava crackers matches) and
obtained values of 7,25 ± 3,8640 levels mg / kg. Based on these results,
Rhodamine B still found in crackers that sold in the market Antasari
Banjarmasin.
Keywords: crackers, Rhodamine B, Thin Layer Chromatography, and
UV-Vis spectrophotometry
Penulis: Eka Kumalasari
Kode Jurnal: jpfarmasidd150525
