IDENTIFIKASI SENYAWA AKTIF FRAKSI ETANOL DAUN SIRSAK (Annona muricata Linn.) SEBAGAI INHIBITOR α-AMYLASE
Abstract: Diabetes mellitus
(DM) is a chronic metabolic interference characterized by both postprandial and
fasting glycemic increase with disturbances in carbohydrate, fat and protein
metabolism. There has been much interest in the development of alternative
medicine for type 2 diabetes. Annona muricata or sirsak has been use to cure
cancer and according to American Association of Clinical Endocrinologist there
was a significant increasing for patient cancer with obesity and diabetes. The
goal of the present study was to provide in vitro evindence and chemical
structure for potential inhibition of α-amylase. This was performed using 𝛼-amylase
from Aspergillus oryzae and the result was Ethanol fraction from Annona
muricata leafes gave the highest inbitory activity against 𝛼-amylase
(IC50 73,54 ppm) furthermore inhibition of isolates compounds Fr.EtOH.4.2.3
gave IC50 0,12 ppm of 𝛼-amylase. Analysis data
and identification of active compounds is done by LC-MS and FT-IR,
identification active compound of 𝛼-amylase inhibitors is
mixture of Muricatin C, cis-Reticulatacin-10-one and 3-Methylquercetin 7-[galactosyl-(1->4)-glucoside].
Keywords: Diabetes, α amylase,
annona muricata, sirsak
Penulis: Eko Mugiyanto,
Partomuan simanjuntak, Siswa Setyahadi
Kode Jurnal: jpfarmasidd170455
