INHIBITORY ACTIVITY OF ENDOPHYTIC FUNGI ISOLATED FROM SUKABUMI TURMERIC PLANT (Curcuma longa L.) AGAINST MCF-7 CELL LINE
Abstract: Kunyit or turmeric
plant (Curcuma longa L.) is a native Southeast Asia plant that has been widely
used as herbal medicine. In our previous research we isolated and screened 44
of endophytic fungi of turmeric plant from Sukabumi and Cibinong to determine
their antioxidant activity. There were 4 samples isolated from Sukabumi with
antioxidant activity for more than 70% at concentration of 100ppm, K.Cl.Sb.R9
(93%), K.Cl.Sb.A11 (81%), K.Cl.Sb.B1 (79%) and K.Cl.Sb.R11 (71%). This research
aimed to determine inhibitory activity of the endophytic fungi against estrogen
positive MCF-7 breast cancer cell line. To obtain the filtrate, the broth
cultures were filtered and the extracellular fraction was extracted with ethyl
acetate. The inhibitory activity was determined by using MTT assay. The result
showed that the ability of endophytic fungi to inhibit MCF-7 growth was dose
dependently. IC50 of endophytic fungi K.Cl.Sb.R9 was 579±38 ppm, K.Cl.Sb.R11
was 542±21ppm, K.Cl.Sb.B1 was 446±15ppm and K.Cl.Sb.A11 was 520±28ppm.
Fluorescent double staining based method using calcein AM and ethidium bromide
was performed to confirm the inhibitory activity. At 500ppm of filtrate
concentration with 24h of cell treatment, treated cell lines showed fewer
viable cells compared to untreated cell lines. The four isolates of endophytic
fungi were able to inhibit proliferation of human breast cancer MCF-7 cell
line.
Keywords: MCF-7; inhibitory
activity; endophytic fungi; turmeric plant
Author: Ratih Asmana Ningrum,
Bustanussalam, Popi Hadi Wisnuwardhani, Neng Herawati, Adi Santoso, Partomuan
Simanjuntak
Journal Code: jpfarmasigg170008
