ISOLASI DAN IDENTIFIKASI GOLONGAN KOMPONEN KIMIA AKTIF ANTIBAKTERI EKSTRAK N-BUTANOL SAWO MANILA (Achras zapota LINN.) ASAL KOTA PALOPO
Abstrak: Sawo manila is one of
the Zapotaceae family plant which have been investigated on n-butanol extract
generate active spot as an antibacterial, therefore isolation and investigation
of which is necessary. The aim of the
investigation was to identify the chemical group of antibacterial active chemically. The methanol extract
obtained by maceration method was 50 gram, continued by partition with diethyl
eter obtained 17,3 gram dried extract, the residue ofiz then partition with n-butanol
obtained 3,7 gram dried extract the extract of which isolated by conventional
colomn cromotography method obtained 7 fractions. The second fraction on
Bioautografi-TLC given antibacterial activity. Then the fraction re-isollated
by preparative-TLC gained the second (2nd) band of which crystalzed by
two dimention of TLC and multi eluen system. The single spot had positive
reaction on the spot visualizer was alkaloid group with the maximum wavelength
on 295,5 nm and the infra red spectra interpretation shown that amin group (NH)
on wave value 1247 cm-1, 2361 cm-1, and 1294 cm-1 strengthen by secondary amin
functional group (-NH-), within wave value 3447 cm-1 and 1294,26 cm-1, aromatic CH group and the aromatic amin 1451
cm-1, unconjugated alkene group on 1652.32 cm -1 strengthen by alkene group on
2922,37 cm-1.
Key words: Antibakterial, Sawo Manila, Achras zapota
Linn.
Penulis: Rusli, Dedy Prayitno
Kode Jurnal: jpfarmasidd170420
