ISOLASI SENYAWA FLAVONOID EKSTRAK BIJI PINANG MERAH (Areca vestiaria Giseke) DAN UJI SITOTOKSIKNYA MELALUI UJI BRINE SHIRIMP LETHALITY TEST (BSLT)
Abstract: Cytotoxic test is
one method to predict the development of compounds that are toxic to the cell
that is an absolute requirement for anticancer drugs. The aim of this study is
to isolate flavonoids and determine LC50 value of the activity of the Red
Pinang seed extract (Areca vestiaria Giseke). Extracts prepared by maceration
method using ethanol 96%. Isolation is done through a range of methods includes
partitioning using n-hexane, a preliminary test using the color reagent and
KLT, isolation by preparative thin layer chromatography, thin layer
chromatography multi eluent, UV-Vis spectrophotometer analysis and cytotoxic
test isolates. The results showed that isolates flavonoid seed extract of Red
Pinang (Areca vestiaria Giseke) has the medium potential of being a cytotoxic
agent with LC50 values of 583.445 ppm.
Keywords: Seed of Red Pinang
(Areca vestiaria Giseke); flavanoid; cytotoxic; Artemia salina Leach
Penulis: Febriyanto Rerung,
Syariful Anam, Akhmad Khumaidi
Kode Jurnal: jpfarmasidd170555
