Isolation of Secondary Metabolite A. niger “In-Habiting” Queen M. gilvus Hagen.’s Nest
Abstrak: Aspergillus niger is
pathogen fungi that can live in various locations and can live contiguous with
many hosts, one of them is queen termite’s nest. The aims of the study were to
isolated the secondary metabilite of A.niger. Extraction proccess of secondary
metabolite compounds was carried out by maceration method using methanol
solvent. Based on that proccess, methanol extract was be yield 4,32% sample
weight. Fractination proccess was carried out in the separating funnel using
ethyl acetate solvent, which ethyl acetate fraction was be yield 14.39%
methanol extract. Separation of the compounds was carried out by column
chromatography method using n-hexane and ethyl asetate eluents. Purification of
the compounds were done by recrystallization method using n-hexane and ethyl
asetate. Two secondary metabolite compounds were successfully isolated from
ethyl acetate fraction of the methanolic extract of fungus A. niger
“In-Habiting” queen termite’s nest M. gilvus Hagen. Based on organoleptic
examination, the compound signed AM-12-22-01 is 35 mg, white needle crystals,
melting point 151-153 oC. While, the AM-12-60-01* is 15 mg, white needle
crystals, melting point 91-93 oC. Based on the chemical analysis, thin layer chromatography,
ultraviolet and infrared spectra data it was identified that AM-12-22-01 and
AM-12-60-01 were a phenolic compounds.
Key words: isolation, A.
niger, In-Habiting, M. gilvus Hagen
Penulis: Yohannes Alen, Atika
Melati, Gemmy Sarina, Akmal Djamaan
Kode Jurnal: jpfarmasidd180226
