KANDUNGAN BETA KAROTEN DAN AKTIVITAS PENANGKAPAN RADIKAL BEBAS TERHADAP DPPH (1,1-difenil 2-pikrilhidrazil) EKSTRAK BUAH BLEWAH (Cucumis melo var. Cantalupensis L) SECARA SPEKTROFOTOMETRI UV-VISIBEL
Abstract: Cantaloupe (Cucumis
melo var. Cantalupensis L.), a fruit containing some compounds, have activity
to protect human body from free radical. The beta carotene (provitamin A) was
recognized as antioxidant compound. Antioxidants could protect the body from
cardiovascular damage caused by free radical.
The aims of this study was to determine the levels of beta carotene and
antioxidant activity (IC50) from Cucumis melo var. cantalupensis L. extract
using DPPH (1,1-diphenyl-2-picrylhydrazyl) method. Cantaloupe was extracted using
n-hexan-acetone-methanol ratio (2: 1: 1). Identification of beta carotene was
performed using 25% antimony trichloride reagent followed by TLC and UV-Vis
spectrum test compared with beta carotene as standard. The content of beta
carotene and free radical scavenging activity was measured
spectrophotometrically at 425 nm. The beta carotene level of cantaloupe was
3.171 ± 0.150 %. The antioxidant activity, presented as IC50 value of
cantaloupe extract, was 12.137±0.44 µg/mL and the antioxidant activity of
standard beta carotene was 2.15 ± 0.172 µg/mL. The cantaloupe extract contained
beta carotene compunds but the antioxidant activity of extract was lower than
beta carotene standard.
Keywords: cantaloupe; beta
carotene; DPPH
Penulis: Aprilia Kusbandari,
Hari Susanti
Kode Jurnal: jpfarmasidd170442
