Optimasi Formula Gel Antioksidan Ekstrak Etanol Buah Bligo (Benincasa hispida) dengan Metode Simplex Lattice Design (SLD)
Abstract: Benincasa hispida
has many benefits including as antioxidant. This study aims to evaluate the
effect of Carbomer 940, HPMC and NaOH concentrations in antioxidant gel formula
and to determine Carbomer 940, HPMC and NaOH concentrations that could produce
the optimal antioxidant gel formula. Benincasa hispida was extracted by
maceration method and antioxidant activity was tested by DPPH method. The
antioxidant gels were formulated with variety combinations of Carbomer 940,
HPMC and NaOH. The optimal gel formula was obtained by Design Expert 7.1.5 with
Simplex Lattice Design method. The physical characteristic of optimal gel
formula was retested and compared with the results of Simplex Lattice Design
.The results showed that antioxidant activity of Benincasa hispida ethanolic
extract has potent activity with the IC50 40,28 μg/mL. The increasing
concentration of Carbomer 940, HPMC and NaOH possessed the high coefficients
viscosity (cPs) with the value of 4366, 4133 and 3750 respectively. The
increasing concentration of NaOH possessed pH and dispersive power of the gel
with coefficients of 11,0 and 5,21 respectively. The desirability values
suggested by Simplex Lattice Design were 1,000 with component variation of the
optimal formula were Carbomer 940 1,5%, HPMC 0,5% and NaOH 0,4%. The
significance test of the optimal formula
compared to the results of laboratory experiments showed the
significance of viscosity of 0,130, the dispersive power of 0,348 and pH of
0,184 that means there was no significant difference between the prediction
values by using Simplex Lattice Design and results of laboratory experiments.
It was indicated that significance values of each responses were over 0,05
(p>0.05).
Keywords: Benincasa hispida, Antioxidant,
Gel, Optimation, SLD
Penulis: Suryani, Andi
Nafisah, Syahrir Mana'an
Kode Jurnal: jpfarmasidd170579
