OPTIMIZATION OF MIXING TEMPERATURE AND SONICATION DURATION IN LIPOSOME PREPARATION
Abstract: Liposomes are a
delivery system used in pharmaceutical products and cosmetics. Liposomes have
many advantages such as increase stability and efficacy, can be targeted to
reduce toxicity and increase accumulation at the target site and are
biocompatible. Preparation of liposomes
can be done by conventional or new methods which are still being developed.
Conventional methods often require a long time and organic solvents which may
be toxic. Heating (Mozafari method) is one of the new methods developed in the
manufacture of liposomes without organic solvents. Mixing temperature can
affect the physical properties of liposomes. The particle size has become one
of the important physical properties because it affects the absorption of the
drug. Sonication is an easy method of choice in reducing the size of liposomes.
Optimization of mixing temperature and duration of sonication in liposomes’
preparation using new heating methods and sonication were performed by
factorial design with 2 factors and 3-levels to obtain optimal liposome size.
Data were analyzed with two-way ANOVA. The results showed that both mixing
temperature and sonication duration significantly affect liposome size, but the
interaction was not statistically significant. Data analysis also showed that
mixing temperature, sonication, and their interaction do not affect the
polydispersity index of liposome. Results showed the optimum mixing temperature
and sonication duration that can produce liposomes with size below 100 nm is at
60°C for 30 minutes.
Keywords: duration; liposome;
mixing; sonication; temperature
Penulis: Dina Christin Ayuning
Putri, Rini Dwiastuti, Marchaban Marchaban, Akhmad Kharis Nugroho
Kode Jurnal: jpfarmasidd170459