PENENTUAN WAKTU OPTIMUM PRODUKSI METABOLIT SEKUNDER ISOLAT BAKTERI ACTINOMYCETES DARI TANAH RHIZOSFER AKAR TANAMAN JARAK PAGAR (Jatropha curcas. L) TERHADAP BAKTERI PATOGEN
ABSTRACT: The study of optimum
time determination of secondary metabolite production of Actinomycetes bacteria
isolate from Jatropha curcas L root of the soil (Jatropha curcas (L) of
pathogenic bacteria has been done with the aim to determine pathogen bacteria
that can be inhibited by Actinomycetes bacteria and determine the optimum time
in producing secondary metabolite to pathogenic bacteria. The first phase of
this study is to isolate Actinomycetes purification IBPT 01. Then performed a
screening test of activity against pathogenic bacteria. Furthermore, the timing
of production of antibiotics or secondary metabolites with bacterial
fermentation Actinomycetes IBPT 01 begins on the 1st day until the 28th day and
carried out the activity of antibiotics for 28 days. The results obtained in
the screening test Actinomycetes bacteria activity IBPT code 01 provides
antibacterial activity with category which has a diameter of 20 mm 11- barriers
against 8 bacteria while one bacterium with diameter> 20 mm including strong
category. Optimization results showed that the isolated bacteria Actinomycetes
capable of producing secondary metabolites on 9 day, 8 day, 7 day, 4 day and 26
day. From this research can be concluded that the bacterial isolates of
Actinomycetes IBPT 01 can provide the optimum time to produce different
secondary metabolites.
Kata Kunci: Actinomycetes,
Metabolit Sekunder, Waktu Optimum
Penulis: Fitriana, Rusli
Kode Jurnal: jpfarmasidd180149
