PENGARUH KOMBINASI EKSTRAK ETANOL HERBA CECENDET (PHYSALIS ANGULATA L.) DENGAN BEBERAPA ANTIBIOTIK TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN KLEBSIELLA PNEUMONIE
Abstract: Infectious disease
is one of the biggest health problems not only in Indonesia, but worldwide.
Bacteria are important agents in causing infectious diseases. Indonesian people
often use antibiotics along with herbal medicines. Cecendet (Physalis angulata
L.) is one of the traditional plants that have antibacterial activity and
widely used by the Indonesian community. This study aims to test the
antibacterial activity of cecendet herbs and their effects when used in
combination with various antibiotics. The preparation of P.angulata extract was
carried out using reflux method with ethanol 50%. The characteristic
examination and phytochemical screening are examined on simplicia and the
extract. Determination of antibacterial activity from P.angulata extract was
done by microdilution test method by assessing minimum inhibitory concentration
(MIC). Determination of the effectiveness of combination of antibiotics with
herbal cecendet against test microbes Staphylococcus aureus and Klebsiella
pneumoniae was done by using checkerboard method. The results of phytochemical
screening showed that simplicia and P.angulata extract contained alkaloids,
flavonoids, saponins, polyphenols, monoterpenoids, sesquiterpenes, steroids and
triterpenoids. The result of antibacterial activity test showed that P.angulata
extract had antibacterial activity against S.aureus and K.pneumoniae with the
MIC was 128 μg/mL, 256 μg/mL. The synergistic interaction of S.aureus is
demonstrated by the combination of P.angulata herb extract with tetracycline.
The additive/indifferent interaction of S.aureus is demonstrated by the
combination of P.angulata extracts with ampicillin, and against K.pneumoniae
shown by the combination of P.angulata extract of cecendet with ampicillin or
tetracycline. Ethanol extract of P.angulata has antibacterial activity and and
combination with antibiotics (ampicillin/tetracyclin) can give synergistic
effect or additive of S.aureus and K.pneumoniae bacteria.
Keywords: Cecendet, Physalis angulata L., MIC,
antibiotic combination
Penulis: Anna Choirunnisa,
Afifah Bambang Sutjiatmo
Kode Jurnal: jpfarmasidd170314
