Pengembangan dan Validasi Metode Analisis Betametason dalam Tablet dengan Metode Absorbansi dan Luas Daerah di Bawah Kurva Secara Spektrofotometri Ultraviolet
ABSTRACT: A precise, accurate,
fast and economical method has been developed and validated for the analysis of
betamethasone tablets using ultraviolet spectrophotometry with absorbance
method and area under the curve. Determination of this method was carried out
at maximum wavelength absorption of 241.40 nm and area under the curve in the
range 210.40 - 290.40 nm using a 0.1 N hydrochloric acid solvent. The method of
analysis was validated by linearity, detection limits, quantification limits,
precision and accuracy. The linearity in absorption method shows the result of
regression equation y = 0.02273 x + 0.00228 with r = 0.99958, with method of
area under curve shows result of regression equation y = 0.643 x – 0.214 with r
= 0.9955. Limit detection and quantification limits on absorbance method
1.754531 and 5.316812, with area wide method under curve 3.888631 and
11.783730. Accuracy obtained by the absorbance method and the area under the
curve satisfy the validated requirements of 80-120%. The precision obtained by
the absorbance method and the area under the curve satisfies the validated
requirement with the RSD yield ≤ 2%. Statistical analysis showed that both
methods did not differ significantly (P ≥ 0.05).
Kata kunci: betametason; metode absorbansi; metode luas daerah di bawah
kurva
Penulis: Harrizul Rivai, Widya
Astuty, Ridho Asra
Kode Jurnal: jpfarmasidd170138
