Pengembangan Dan Validasi Metode Analisis Propanolol Hidroklorida Tablet Dengan Metode Absorbansi Dan Luas Daerah Di Bawah Kurva Secara Spektrofotometri Ultraviolet
Abstrak: The simple, accurate,
sensitive and selective ultraviolet spectrophotometry has been developed and
validated for analyzing of propanolol hydrochloride tablets with absorbance and
area under curve method. These methods used solvent HCl 0.1 N with wavelength
288.60 nm, and the area under the curve measured at a wavelength of 248-302.80
nm. The calibration curve obtained in the concentration range of 8-16 µg/mL.
With absorbance method showed results of the linear regression equation of y =
- 0.1516+0.0502x and value of r = 0.99993
and with the area under curve method showed results of the linear regression
equation y = –2.664+0.7657x and value of r = 0.99973. The percentage of
propanolol hydrochloride tablets with trademark of propanolol HCl absorbance
method was 99.21 % and with area under
curve method it showed 101.24 %. The percentage levels of both samples fill the
Indonesian Pharmacopoeia standards edition V which was 90–110 %. The average of
percent recovery and RSD were obtained from both samples by absorbance and area
under curve fill of validation parameters, ie 80-120 % and 1-2 % respectively.
Statistical analysis showed that there was no significantly different between
both of these method (sig.>0.05).
Kata Kunci: Area Under Curve
Methode; Propanolol Hydrochloride; Ultraviolet Spectrophotometry
Penulis: Boy Chandra, Harrizul
Rivai, Edwin Apriansyah
Kode Jurnal: jpfarmasidd170357
