PERBANDINGAN KADAR ASIATIKOSIDA DALAM EKSTRAK ETANOL 70% PEGAGAN (Centella asiatica (L)Urban) DENGAN METODE EKSTRAKSI MASERASI DAN SONIKASI SECARA LC-MS/MS
Abstrak: Currently, among the
many new types of modern medicine in the market there is a global tendency for
going back to nature. In Indonesia, for generations plants that have medicinal
properties have been used based on the ancestors experience. One of the plants
oftenly used as a medicine is pennywort (Centella asiatica (L) Urban). One of
the major component of pennywort is asiatikoside which has activity to
accelerate wound healing. The objective of this study was to compare the
extraction method that produce the highest levels of asiatikoside in 70%
ethanol extract of pennywort. The extraction method were maceration and
sonication in ethanol 70%. Qualitative analysis of asiatikoside was performed
by thin layer chromatography (TLC) producing purplish stains after being soaked
in anisaldehid sulfuric acid solution and observed under UV lamp at 366 nm. The
results showed at the Rf values 0.45 in maceration and 0.43 in sonication.
Quantitative analysis for assay asiatikosida using LC-MS/MS. Before
quantitative analysis, validation method needs to be done. Results of the
validation are: the linearity have correlation coefficient (R2) of 0.9989, LOD
and LOQ at 0.06 ppm and 0.19 ppm, accuracy value (% recovery) between
98.02%-107.49%, precision value (% Coefficient of Variation) between
0.32%-3.31%, and selectivity (Retention Time) between 3.44-3.49. Asiatikoside
assay results in maceration extract at 7.19% and sonication at 7.43%. The
results of the validation are eligible, so that asiatikoside level were
obtained by LC-MS/MS declared accurate, specific, and precise.
KATA KUNCI: pennywort
(Centella asiatica (L) Urban)., asiaticoside., maceration-sonication., TLC.,
LC-MS/MS
Penulis: Alifia Putri
Febriyanti, Siti Jazimah Iswarin, Tristy Digjayanti
Kode Jurnal: jpfarmasidd160702
