PHARMACODYNAMICS STUDY OF ETHANOL EXTRACT OF CYCLEA BARBATA (MIERS.) LEAVES ON SRF AND COX-2 GASTRIC MICE WITH NSAID GASTROPATHY
Abstract: Epidemiology of
NSAIDs gastropathy is increasing as increase number of usage. From arthritis to
cardiovascular events and cancer prevention, the versatility of NSAIDs is not
questioned. However, no dose of NSAIDs is safe. No matter low dose, or single
use, NSAIDs will cause gastric damage upto 3-7 days after use. In inflammation
and healing process of gastropathy there are various proteins involved, but
treatment with COX-2 and SRF are associated with an immediate healing and
better quality of gastric mucosa. Cyclea barbata (miers.) has been declared as
functional food for preventing and treatment gastropathy yet, its mechanism of
actions have not yet clearly discovered. Hence the aim of this study is to
analyze the effects of Cyclea barbata (miers.) ethanol extract to COX-2 and SRF
in gastric tissue, in time series basis. Laboratory mice was induced with
aspirin to produce gastropathy and then treated with Cyclea barbata (miers.)
extract for 1, 3, 7, 10 or 14 days. Gastric tissue then harvested and analysed
with elisa procedure to determine tissue SRF and COX-2 level. Treatment was
proven to increase COX-2 and SRF higher than control group. This concludes one
of Cyclea barbata (miers.) mechanism for NSAIDs gastropathy is by increasing
tissue COX-2 and SRF.
Keywords: Aspirin-induced gastropathy,
COX-2, SRF, NSAIDs
Author: Florence Pribadi, Suhartati, Achmad Basori
Journal Code: jpfarmasigg170020
