Preparation and Characterization of Ba-Alginate Microspheres Containing Ovalbumin
Abstract: The use of
microspheres nowadays is important in the pharmaceuticals formulation. The aim
of the present research was to evaluate physical characteristics of
ovalbumin-loaded alginate microspheres using aerosolisation technique at
various concentrations of alginate and BaCl2. Alginate microspheres were
characterized in terms of encapsulation efficiency, yield, particle size,
surface morphology and protein integrity. The comparative study between
concentrations of BaCl2 and alginate polymer was investigated. Increasing
concentration of alginate polymer from 2.5 to 3.5% reduced their particle size
and formed smoother and spherical microspheres. Increasing Ba2+ concentration,
simultaneous increased the encapsulation efficiency and yield. Scanning
electron microscope (SEM) photomicrographs revealed that with the increase in
the electrolyte concentration the density of the cross link is also increased.
Smoother surface was demonstrated when the electrolyte concentration is
increased from 0.5M to 0.75M. Formula prepared using 0.75M BaCl2 and 3.5%
alginate polymer resulted the highest encapsulation efficiency (92%), the
highest microspheres yield (73%) and the smallest microspheres size (3.73µm).
Most of the formulations maintain the integrity of ovalbumin after 2 hours
incubation in pH 1.2 followed by 8 hours incubation in pH 7.4. The different
formulations produced different physical characteristics of ovalbumin-loaded
alginate microspheres.
Keywords: alginate, BaCl2,
microspheres, integrity, protein
Penulis: Dewi Melani Hariyadi,
Esti Hendradi, Mukhlis Bayu Irawan
Kode Jurnal: jpfarmasidd160630
