Quantitative Analysis of Bioactive Compounds in Extract and Fraction of Star Fruit (Averrhoa carambola L.) Leaves using High Performance Liquid Chromatography
Abstract: Star fruit (Averrhoa
carambola L.) is potential as raw material for medicine, native in tropic
areas, including Indonesia. According to other study report, star fruit leaves
containing flavonoids apigenin and quercetin as potential anti-inflammatory and
anticancer agents. The raw material for the drug in Indonesia mostly obtained
through imports from other countries. In order to support the independence of
traditional medicine raw materials, it is important to standardize the quality
of traditional medicine raw materials, in this case is star fruit leaves by
High Performance Liquid Chromatography (HPLC) method. The sample used is star
fruit leaves extract obtained from maceration process using ethanol 70%; water
fraction, ethyl acetate and hexane fractions obtained from fractionation
process of the ethanolic extract. Physical parameters analyzed in sample
include appearance, color, odor, taste, extract yield, water content, loss of
drying, total ash content, residual solvent. Chemical parameters analyzed
include apigenin and quercetin contents. The results shows that star fruit
leaves used in this study meet the standards of Indonesian Herbal Pharmacopoeia
with highest apigenin (6.37%) and quercetin (4.49%) content are in ethyl
acetate fraction.
Keywords: Apigenin; Quercetin;
Averrhoa carambola L; HPLC
Penulis: Nanang Yunarto, Novi
Sulistyaningrum
Kode Jurnal: jpfarmasidd170539
