SIMULTANEOUS DETERMINATION OF SODIUM BENZOATE AND SODIUM CYCLAMATE IN SOFT DRINK USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
ABSTRACT: Sodium benzoate and
sodium cyclamate are foods additives generally used as preservative and
artificial sweeteners, respectively. Monitoring of sodium benzoate and sodium
cyclamate need appropriate analytical method. Objective: The main objective of
this study was to gain the optimum conditions for simultaneous determination of
sodium benzoate and sodium cyclamate in soft drink by High Performance Liquid
Chromatography. Methods: Measurement of the additives was performed in
ยต-Bondapak RP18 column using methanol:phosphate buffer of pH 4 (42:58) as
mobile phase with flow rate of 1 mL/minute. Results: It has shown that
correlation coefficients (r) of sodium benzoate and sodium cyclamate was 0.9997
and 0.9991 respectively. Recoveries of the two analyst were acceptable.
Conclusions: Five out of nine samples tested contained sodium cyclamate higher
than the recommended concentration. All sodium benzoate containing samples were
within the recommended concentration.
KEYWORDS: sodium benzoate;
sodium cyclamate; soft drink; high performance liquid chromatography
Penulis: Ariya Wijaya Putra,
Asri Darmawati, Juniar Mochtar
Kode Jurnal: jpfarmasidd160761
