Skrining Aktivitas Antibakteri Bakteriosin dari Minuman Ce Hun Tiau
Abstract: Lactic acid bacteria
is one of the beneficial bacteria because it can produce an antimicrobial
peptide called bacteriocin. Bacteriocin has been applied as a natural food
preservative because it prevents effectively the growth of pathogenic bacteria
on food or drink. The aimed of this study was to identify the lactic acid
bacteria as producers of bacteriocin from Ce hun tiau which has inhibitory
activity againts pathogenic bacteria such as Escherichia coli, Staphylococus
aureus and Salmonella typhi. In this study, Lactic acid bacteria were isolated
from Ce Hun Tiau using streak plate method on media deman Rogose Sharpe.
Screening bacteriocin use disc diffusion method to find out the inhibitory activity
of isolates against Escherichia coli, Staphylococus aureus and Salmonella typhi
and for confirmation test using proteolytic enzymes. The results showed one
isolate of lactid acid bacteria was succesfully isolated from Ce hun tiau,
identified as Lactobacillus plantarum. This isolate could inhibit the growth of
pathogenic bacteria Escherichia coli, Staphylococus aureus and Salmonella typhi
which is a bacteriocin activity.
Keywords: bakteriosin; bakteri
asam laktat; ce hun tiau; bacteriocin; lactic acid bacteria; ce hun tiau
Penulis: Rafika Sari , Lia
Deslianri , Pratiwi Apridamayanti
Kode Jurnal: jpfarmasidd160716