The Activities of Antifertility Ethanol Extract Guava Leaves (Psidium Guajava L.) based on The Analysis Of Cement and Display of Immunohistochemistry Cyclooxygenase-2 In Testis Of Mice (Mus Musculus L.)
Abstract: Population growth
increase, without support from good economic conditions, requirer population
control. One of the program to control population growth is family planning
program (KB). The participation of men in family planning programs is very
important. Male contraception can utilize natural materials derived from
plants. One of the herb is guava leaves (Psidium guajava L.) which contains
many active substances that can inhibit the process of spermatogenesis. The
purpose of this study was to determine the effect of ethanol extract guava
leaves to the quality (concentration, viability, morphology, and motility) of
spermatozoa and the expression of Cyclooxygenase-2 (COX-2) in the testes of
mice (Mus musculus L.). The research used experimental design with completely
randomized sampling method. Four treatment groups, namely P0 using aquadest as
a control 0,5 mL, P1 using 15 mg/25g body weight extract orally, P2 using 20
mg/25g body weight extract orally, and P3 using 25 mg/25g body weight extract
orally. Data were analyzed using One Way ANOVA followed by Bonferroni Post Hoc
test 5%. The results showed that ethanol extract guava leaves with a dose of
15mg/25g body weight had significantly decreased sperm quality and increased
expression of COX-2 in the testes of mice. This research indicates that guava leaves
have a potential to be used as a herbal contraceptive.
Keywords: Guava leaves,
contraceptive, sperm quality, COX-2
Penulis: RICA VERA BR TARIGAN
Kode Jurnal: jpfarmasidd160412
