The Antihyperglycemic Activity of Ethanol Extract of Trigonella foenum-graecum L. and Its Effect on the GLUT-2 Expression of Streptozotocin-NicotinamideInduced Rats

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ABSTRACT: Insulin is an important factor in glucose transport. Glucose transporter (GLUT-2) in pancreatic β cell membranes isresponsible for glucose transport that will stimulate insulinsecretion. The purpose of this research was to investigate theantihyperglycemic activities of extract of Trigonella foenumgraecum L. (fenugreek) seed and the effects on GLUT-2 protein expression in pancreatic β cells on Streptozotocin-Nicotinamideinduced (STZ-NA) rats. This research utilized five groups of male Wistar rats. Group I was normal control group, while Groups II-Vwere induced with STZ-NA. Group II was negative control group(CMC Na 1%); group III was positive control group(Glibenclamide); group IV and V was examined group (fenugreekseed ethanol extract of 100mg/kg bw and 200 mg/kg bw)). Thetest compounds were orally administered for 14 days.Measurement of blood glucose levels and observation of theexpression of GLUT-2 protein in pancreatic β cells in rats wereconducted by immunohistochemistrical method. The researchresults revealed that the examined group dose of 200 mg/kg bwof fenugreek seed extract appeared to show blood glucose leveldecline that was proportionate to that of the positive controlgroup. The increased density of GLUT-2 protein in pancreatic βcells significantly occurred in the group injected with 200 mg/kg bw compared to the group injected with 100 mg/kg bw. Hence, 200 mg/kg bw of fenugreek seed ethanol extract was able to  decrease blood glucose levels and increase the GLUT-2 protein expression in pancreatic β cells in STZ-NA-induced rats with theeffectiveness that was comparable to the positive control group.
Keywords: Trigonella foenum-graecum L, Streptozotocin-Nicotinamide, Glucose transporter-2
Author: Jena Hayu Widyasti, Gunawan Pamudji Widodo, Rina Herowati
Kode Jurnal: jpfarmasigg180003

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