The Effect of Extraction Condition on The Polyphenol Content and Antioxidant Activity of Curcuma zedoaria (Christm.) Roscoe Rhizome
Abstrak: White turmeric
(Curcuma zedoaria (Christm.) Roscoe) is one of Indonesian herbal medicine. The
extraction process to get active compound was influenced by some factor such as
solvent, temperature, time, and method of extraction. The objective of this
study was to determine the significant factor of extraction that is solvent, temperature and time of
extraction on the polyphenol content and antioxidant activity of white turmeric
(Curcuma zedoaria (Christm.) Roscoe) rhizome. Extraction was done by dynamic
maceration method with variations (23 factor variable design) of solvent
(ethanol 95% and water), temperature (25°C and 70°C), time (6 and 24 hours).
The method of analysis of polyphenol content using Folin Ciocalteu reagent, and
the antioxidant activity using DPPH free radical reduction method. The experiment
design and data analysis using software Design Expert ver.10. The result showed
that extraction using ethanol 95% at 70°C for 24 hours was gave high polyphenol
content and antioxidant activity. Data analysis was showed that polyphenol
content and antioxidant activity was influenced only by solvent of extraction.
This study indicated that solvent is significant extraction factor for
polyphenol content and antioxidant activity of white turmeric (Curcuma zedoaria
(Christm.) Roscoe) rhizome.
Keywords: Antioxidant, Curcuma
zedoaria (Christm.) Roscoe, extraction, polyphenol
Penulis: Lia Marliani, Wempi
Budiana, Yonara Anandari
Kode Jurnal: jpfarmasidd170604