The Immunosuppressant Effect Comparation Between Ethyl Acetate and n-Butanol Fractions of Kalanchoe Pinnata (Lmk) Pers In 2,6,10,14 Tetramethylpentadecane-Treated Mice
Abstract: Immunosuppressant
drugs are the main treatment of lupus patient. The ACR and SLICC treatment
guidelines are able to increase the quality of life, but the outcome is not
satisfying since the off-label therapy of corticosteroids and cytotoxic drugs
give a lot of side effects. Many breakthrough efforts still develop in order to
find the safe and effective drugs for lupus, such as finding immunosuppressant
drugs from natural resources. One of the potential resources is Kalanchoe
pinnata (Lmk) Pers, which have immunosuppressant, anti-inflammatory,
antinociceptive, and antioxidant effects. Thus, in the previous study, we found
the effect of the aqueous extract of Kalanchoe pinnata (Lmk) Pers is active to
repair the lupus manifestation in 2,6,10,14 tetramethylpentadecane
(TMPD)-treated mice. Then, this research was focused on the in vivo
immunosuppressant effect of a flavonoid-rich fraction of the extract which was
consisted of the ethyl acetate (FE) and n-butanol (FB) fractions. The induction
method and the extraction procedure were the same as the previous study and
then the fractionation was performed by using liquid-liquid extraction. After
2-week treatment of both fractions, we obtained the differences in the total
leukocytes, organ indexes, and also the spleen, kidney, and joint structure
parameters. The total leukocyte of the FE group was 3,600±264 cells/mm3, which
was lower than that in the FB group. The spleen and kidney indexes increased
after the administration of FB fraction, while the FE fraction was not. At
last, despite the histology observation of spleen resembled mild structural
changes differences, the clear differences between both treatment groups
occurred in the kidney and joint histology. The differences led to a conclusion
that the FE fraction has the better immunosuppressant effect in TMPD-treated
mice.
Keywords: lupus model,
Kalanchoe pinnata (Lmk)Pers, flavonoids, ethyl acetate fraction, n-butanol
fraction
Penulis: Niken Indriyanti, Joewono
Soeroso, Junaidi Khotib
Kode Jurnal: jpfarmasidd170161
