UJI AKTIVITAS ANTIMIKROBA HASIL FRAKSINASI EKSTRAK ETANOL DAUN PATIKALA (ETLINGERA ELATIOR) TERHADAP BEBERAPA MIKROBA UJI
Abstrak: The purpose of this
study was to determine the activity of ethanol extract of leaves patikala
fraction (Etlingera elatior) as an antimicrobial in some microbes and identify
the components of compounds in the leaves patikala which acts as an inhibitor
of the microbes. The research was done by using KLT-Bioautografi. Simplicia
leaves patikala macerated with maceration method stratified by using solvent
n-hexane and ethanol 96%, then the test screening of bacteria which then
extract fractionation actively performed by the method of KCV (Vacuum Liquid
Chromatography), chromatographic separation of compounds results then carried
TLC -Bioautografi are placed on the surface of the solidified medium. After
15-30 minutes plates (chromatogram) is appointed and removed from the medium,
were incubated for 1 x 24 hours at 37 ° C for bacteria and 3 x 24 hours at room
temperature for mushrooms. Results showed that the microbes could be reversed
after tested KLT- Bioautografi there are 5 bacterium is Escherichia coli, Bacillus
subtilis, Staphylococcus aureus, Streptococcus mutans, and Pseudomonas
aeruginosa. Further quantitative research to determine the level of
effectiveness of the results of the leaf fraction patikala to determine the
value of MIC (Minimum Inhibitory Concentration) and the sensitivity of the most
active fraction against microbes Escherichia coli, Bacillus subtilis,
Staphylococcus aureus, Streptococcus mutans, and Pseudomonas aeruginosa.
KATA KUNCI: Antimicrobial.,
Fractionation results., KLT-Bioautografi., Flavonoids
Penulis: Faridha Yenny Nonci,
A Tenriugi Daeng, Hasnia A
Kode Jurnal: jpfarmasidd160700
