UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN ARBENAN [Duchesnea indica (Jacks.) Focke] DENGAN METODE DPPH
ABSTRACT: Arbenan [Duchesnea
indica (Jacks.) Focke] is a plant belonging to Rosaceae family which server as
an antioxidant, flavonoids. This research aimed to determine the antioxidant
activity of ethanol extract of arbenan leaves using DPPH. The leaves were
extracted using maceration method with a sample size of 300 gs dissolved using
ethanol 70%, then obtained the viscous extract of 32,49 gs. The arrest of free
radicals for each tested sample under the inhibition of DPPH. The tests were
conducted at the 4 concentration series of 10, 20, 30, and 40 ppm by using
methanol p.a by the addition of 0,5 mL with 3,5 mL DPPH of 30 ppm. Antioxidant
activity absorbance was measured by a spectrophotometer at the wavelength
of 515 nm and IC50 value was calculated
based on the absorbance data. The calculations showed that the Arbenan leaves
have a very strong antioxidant activity with IC50 value of 30,20 µg/mL.
Keywords: Antioxidant, Ethanol extract of arbenan
leaves [Dunchesnea indica (Jacks) Focke], DPPH.
Penulis: Nuraziza, Seniwati
Dali, Risda Waris
Kode Jurnal: jpfarmasidd170417
