UJI DAYA HAMBAT EKSTRAK ETANOL RIMPANG TEMU GIRING (CURCUMA HEY NEANA VAL.) TERHADAP PERTUMBUHAN ESCHERICHIA COLI SECARA IN VITRO
Abstract: Infection is a major
problem that the world’s attention. Infectious diseases have caused the death
of over 13 million people worldwide every year, particularly in the developing
countries such as Indonesia. One of the species of bacteria that cause infections
are Escherichia coli. Curcuma heyneana rhizome (Curcuma heyneana Val.) is a
plant that is often used as a traditional medicine to antibacterial. This study
was intended to determine the antibacterial inhibition activity, and minimum
inhibitory concentrations (MIC) from the usage of an ethanol extract in the
curcuma heyneana rhizome (Curcuma heyneana Val.) for the growth of an
Escherchia coli by in vitro.Curcuma heyneana rhizome (Curcuma heyneana Val.)
was extracted using maseration method with solvent ethanol. Each extracts
identified the active compounds group consisting of flavonoids, saponins,
curcumin, volatile oil, and tannins. This study was an experimental laboratoric
by using the diffusion method with discs blank with 10 treatment groups concentration which are
100%, 50%, 25%, 12,5%, 6,25%, 3,12%, 1,56%, 0,78%, positive control and
negative control with three repetitions.The phytochemical screenings analysis
showed curcuma heyneana rhizome (Curcuma heyneana Val.) of ethanol extract
containing flavonoids, saponins, curcumin, volatile oil, and tannins. Ethanol
extract in the curcuma heyneana rhizome (Curcuma heyneana Val.) had an
effectiveness in inhibiting the growth of an Escherchia coli by in vitro.
Minimum inhibitory concentrations (MIC) from the usage of an ethanol extract in
the curcuma heyneana rhizome (Curcuma heyneana Val.) for the growth of an
Escherchia coli by in vitro is 12,5% with average inhibition zone 9,77 mm. The
results showed that the ethanol extract curcuma heyneana rhizome (Curcuma
heyneana Val.) had an effectiveness in inhibiting the growth of an Escherchia
coli, it showed inhibition zone diameter due to higher concentrations of the
resistance is increasing. Analysis of the test data with Kruskal-Wallis (sig) =
0,001 which is smaller than α < 0,05. It means that there are significant
differences in the average diameter of an each concentration of ethanol extract
of curcuma heyneana rhizome in i
Keywords: Antibacterial, Curcuma heyneana rhizome (Curcuma heyneana
Val.), Minimum inhibitory concentrations
Penulis: Aditya Maulana
Perdana Putra, Rustifah Rustifah, Muhammad Arsyad
Kode Jurnal: jpfarmasidd150523
