VALIDASI METODE ANALISIS DAN PENURUNAN KADAR INFUS CIPROFLOKSASIN YANG DIPENGARUHI REAKSI OKSIDASI MENGGUNAKAN HPLC
Abstract: Validation of
analysis methods is conducted on the assay of the antibiotic ciprofloxacin
because it is often used in the treatment of urinary tract infections, respiratory
tract infections and gastrointestinal infections. This study aimed to determine
the validity of an analytical method. Development of methods used in this study
were the mobile phase of methanol: aquabidest : 0.1 M phosphate buffer:
acetonitrile (80: 10: 5: 5). Then the ciprofloxacin validation was performed.
After the methods validation were valid, the assay of ciprofloxacin in infusion
was performed to determine the decreased levels which affected by the oxidation
reaction of 0, 60, 90, 150 and 180 minutes. The validation parameters in the
research were selectivity, accuracy, precision, linearity, LOD and LOQ. Samples
were standart ciprofloxacin used for the validation and infusion of
ciprofloxacin for the assay on the sample application that was affected by the
oxidation reaction. The used of HPLC met the requirements such as HPLC devices
Waters e2695 Separations, SunFireTM column C18, and PDA detector and a flow
rate of 1.0 mL / min. This study showed that the result was selective, stated
with no emergence of peaks in the matrix. HPLC analysis methods on
ciprofloxacin meet the acceptance criteria of accuracy % recovery value
solution concentration of ≥10 mg / mL are 90-107%. Precision test qualifies %
RSD for solution concentrations ≥10 mg / mL is <7.3%. Linearity r ≥0,997
then it can be concluded that the result was qualified. LOD value is 0.2707 µg
/ mL and the LOQ value is 0.9026 µg / mL. The results of the assay infusion of
ciprofloxacin were found that there was a significant decrease in the levels of
ciprofloxacin due to the influence of the oxidation reaction
Keywords: Validation,
ciprofloxacin, infusion, HPLC, oxidation reaction
Penulis: Bekti Nugraheni, Nafi'ah
Kode Jurnal: jpfarmasidd160469
