Validated TLC Method for Determination of Curcumin Concentrations in Dissolution Samples Containing Curcuma longa Extract
Abstract: Curcumin is a
lipophilic compound which suffers from the poor bioavailability after oral
administration. Increasing its dissolution rate can be a successful strategy to
improve the bioavailability. Along with the formulation developments, a rapid
and simple analytical method to determine curcumin concentrations in the
dissolution medium is required. The aim of this study was to develop and to
validate an analytical method based on thin layer chromatography (TLC) to
determine curcumin concentrations in a dissolution medium containing 0.5% w/v
sodium lauryl sulfate (SLS) and 20 mM sodium phosphate buffer. A
polyvinylpyrrolidone K30 based solid dispersion of Curcuma longa extract and
its corresponding physical mixture were dissolved in a medium containing 0.5%
w/v SLS and 20 mM sodium phosphate buffer (pH 6.0). Dissolution samples were
spotted on a normal TLC plate and eluents of various compositions were
evaluated. The retardation factor (Rf), resolution (Rs), and asymmetry factor
(As0.05) of the optimized method were determined. Using the optimized eluent,
proper separation of curcumin peak was achieved with an Rf of 0.50, Rs of 2.62
and As 0.05of 0.87. Linearity (5-30 μg/mL) was demonstrated by r value of
0.9965. The TLC method provided precision with RSD ≤3.50 and accuracy with
recovery value of 94-105%.
Keywords: Curcuma longa,
curcumin, solid dispersion, dissolution, thin layer chromatography
Penulis: DEWI SETYANINGSIH
Kode Jurnal: jpfarmasidd160421
